➦ PortaCellTec offers three possibilities to measure substrate transporter interaction:
How to identify a test item as a substrate for one specific transporter?
A two-step experimental setup
Time and concentration dependent uptake of a test item is performed in the absence and presence of a probe inhibitor.
Determination of Km and Vmax value: Concentration dependent uptake of a test item to determine the affinity (Km value) and the maximum transport rate (Vmax) of a transporter for a test item.
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